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Please use this identifier to cite or link to this item: http://hdl.handle.net/10225/735

Title:	NANOSTRUCTURED SENSORS FOR IN-VIVO NEUROCHEMICAL RECORDING
Authors:	Silpa, Nagari
Keywords:	Platinized Pt electrodes Platinum black (Pt-Black) L-glutamate H2O2 Glutamate microbiosensor
Date Created:	2007
Abstract:	L-glutamate plays a vital role in central nervous system. It is a neurotransmitter associated with several neurological disorders like Parkinson’s disease, epilepsy and stroke. Continuous and fast monitoring of this neurotransmitter has become a major concern for neuroscientists throughout the world. A simple, sensitive, and reliable L-glutamate microsensor with short response time has been developed using ceramic-based microelectrode arrays with platinum recording sites. The electrodes were modified by electrodeposition of Platinum black (Pt-black) to detect hydrogen peroxide (H2O2) which was produced by enzymatic reactions of glutamate oxidase immobilized on the electrode surface. Modification of Pt electrodes with Pt-black has been adopted because the microscale roughness of Pt-black increases the effective surface area of the electrode and promotes efficiency of H2O2 electro-oxidation. The modified Pt recording sites were coated with m-phenylenediamine (mPD) and L-glutamate oxidase (L-GluOx). mPD acts as an barrier for extracellular interferents such as ascorbic acid and dopamine, thus increasing the selectivity of electrode for Glutamate (Glu). This modified microsensor was highly sensitive to H2O2 (686.3±156.48 μAmM-1cm-2), and Glutamate (492.2±112.67 μAmM-1cm-2) at 700mV versus Ag/AgCl reference. Deposition of Pt nano-particles on recording sites enhanced the sensitivity to H2O2 by 2 times and the sensitivity to glutamate by 1.5 times.
URI:	http://hdl.handle.net/10225/735
Appears in Collections:	Electronic Theses and Dissertations

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