Archive.uky.edu: RELATIONSHIP OF NITROGEN METABOLISM CAPACITY, CARCASS QUALITY, AND EXPRESSION OF GLUTAMATE TRANSPORTERS AND METABOLIZING ENZYMES IN POLYPAY AND PERCENTAGE WHITE DORPER LAMBS

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Please use this identifier to cite or link to this item: http://hdl.handle.net/10225/650

Title:	RELATIONSHIP OF NITROGEN METABOLISM CAPACITY, CARCASS QUALITY, AND EXPRESSION OF GLUTAMATE TRANSPORTERS AND METABOLIZING ENZYMES IN POLYPAY AND PERCENTAGE WHITE DORPER LAMBS
Authors:	Lunsford, Andrea K.
Keywords:	Nitrogen metabolism glutamate transporters glutamate metabolizing enzymes genetic type lambs
Date Created:	2007
Publisher:	University of Kentucky
Abstract:	Two studies were conducted to compare nitrogen (N) and glutamate metabolism in Polypay and percentage White Dorper lambs. First, a two-phase digestion/N metabolism trial was conducted with 18 wether lambs of three genetic types: Polypay (PP), ½ White Dorper ½ Polypay (½ D), and ¾ White Dorper ¼ Polypay (¾ D). Six lambs of each genetic type were fed a high roughage diet (HR; Phase 1) or high concentrate diet (HC; Phase 2). DM and N digestion was higher for ½ D than PP or ¾ D fed HC. N retention was highest for ½ D regardless of diet. The second study analyzed the effect of genetic type on glutamate transporter and metabolizing enzyme expression in liver, kidney, longissimus dorsi muscle (LD muscle), and subcutaneous fat (Sub Q Fat) tissue of 18 wether lambs of three genetic types: PP, ½ D, and 15 ⁄16 White Dorper 1⁄16 Polypay (15 ⁄16 D). Tissue samples were analyzed for protein and mRNA content of GS, GDH, ALT, EAAC1, and GLT-1. Glutamate transport and metabolism capacity was lowest for the heavier muscled 15 ⁄16 D lambs. The results suggest genetic type has an effect on N metabolism due to differential expression of glutamate transporters and metabolizing enzymes.
URI:	http://hdl.handle.net/10225/650
Appears in Collections:	Electronic Theses and Dissertations

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